Nonionic surfactant analysis using HPLC method and their separation
I am getting problems to analyze nonionic surfactants using HPLC method and more over separation is also not good and not reproducible using ACN and water solvent system.
If you have any idea regarding this please write in detail.
Surfactant like Tween are better separated by HPLC using methanol [affil link]. Attention should be paid to problem of a specific retention on column (carry-over).
Separation acid ethoxylate by HPLC. how can I get the oligomers from non-surfactant such as fatty acid ethoxylated by HPLC? I used ACN : H2O system and I just got 1 peak not a oligomers
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